6SEN
TEAD4 bound to a FAM181A peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999820 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.490, 132.070, 62.020 |
| Unit cell angles | 90.00, 115.87, 90.00 |
Refinement procedure
| Resolution | 23.750 - 1.650 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ge3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.224 | 1.732 |
| High resolution limit [Å] | 1.614 | 1.614 |
| Rmerge | 0.038 | 0.835 |
| Number of reflections | 47462 | 2373 |
| <I/σ(I)> | 14.3 | 1.4 |
| Completeness [%] | 77.0 | 20.1 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.999 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.59 | 293 | 200 mM Ammonium sulfate 100 mM Sodium acetate trihydrate pH 4.59 35% Pentaerythritol ethoxylate (15/4 EO/OH) |
