6SEC

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cBon complex with ONPG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-27
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.918400
Spacegroup name	P 31 2 1
Unit cell lengths	137.330, 137.330, 126.784
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.578 - 2.768
R-factor	0.1743
Rwork	0.172
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6etz
RMSD bond length	0.008
RMSD bond angle	1.008
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.578	46.578	2.740
High resolution limit [Å]	2.590	7.660	2.590
Rmerge	0.154	0.027	1.513
Rmeas	0.168	0.030	1.651
Number of reflections	67872	1782	6810
<I/σ(I)>	11.75	55.18	1.03
Completeness [%]	99.4	98.4	98.1
Redundancy	6.625	6.435	6.322
CC(1/2)	0.997	0.999	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	35% Tacsimate pH 6.0