6SE1
Structure of Salmonella ser. Paratyphi A lipopolysaccharide acetyltransferase periplasmic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 93.15 |
| Detector technology | PIXEL |
| Collection date | 2017-04-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.740, 58.430, 90.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.390 - 1.080 |
| Rwork | 0.136 |
| R-free | 0.14930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | null |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.451 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | Fragon |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.390 | 1.100 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Number of reflections | 126378 | 6232 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 7.7 | 6.4 |
| CC(1/2) | 0.999 | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100 mM BisTris pH 5.5, 0.25 M Lithium sulfate, 25% w/v PEG 3350 |
