6SDV
W-formate dehydrogenase from Desulfovibrio vulgaris - Formate reduced form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.885, 128.560, 149.629 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.760 - 1.900 |
| R-factor | 0.1613 |
| Rwork | 0.159 |
| R-free | 0.19650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sdr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.004 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 188388 | 4813 |
| <I/σ(I)> | 7.9 | 1.4 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.994 | 0.484 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 32% PEG 3350, 0.1M Tris-HCl pH 8.5, 1M LiCl |
