6SDF
N-terminal SH3 domain of Grb2 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION SUPERNOVA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-27 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.54184 |
| Spacegroup name | H 3 |
| Unit cell lengths | 97.731, 97.731, 51.032 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.212 - 2.500 |
| R-factor | 0.1621 |
| Rwork | 0.160 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gri |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.391 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.865 | 2.588 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.069 | 0.030 |
| Rmeas | 0.083 | |
| Rpim | 0.045 | |
| Number of reflections | 6312 | 607 |
| <I/σ(I)> | 8.86 | |
| Completeness [%] | 99.9 | |
| Redundancy | 1.892 | 1.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M Hepes, 55% (v/v) MPD |
