6SCQ

Cell Division Protein SepF in complex with C-terminal domain of FtsZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	C 1 2 1
Unit cell lengths	65.840, 32.270, 74.410
Unit cell angles	90.00, 114.65, 90.00

Refinement procedure

Resolution	29.920 - 1.500
R-factor	0.211
Rwork	0.211
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS (version Mar 15, 2019)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.910	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.026	0.166
Number of reflections	21921	1127
<I/σ(I)>	19.6	4.9
Completeness [%]	95.2	97.3
Redundancy	2.6	2.6
CC(1/2)	0.999	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.1 M MES pH 6, 20 %w/v PEG MME 2K, 0.2 M NaCl