6SCF
A viral anti-CRISPR subverts type III CRISPR immunity by rapid degradation of cyclic oligoadenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.829, 51.727, 85.611 |
| Unit cell angles | 80.22, 89.68, 83.38 |
Refinement procedure
| Resolution | 50.640 - 1.550 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x4i |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.635 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.640 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.118 | 0.360 |
| Number of reflections | 119765 | 5590 |
| <I/σ(I)> | 12.3 | 1.7 |
| Completeness [%] | 98.6 | 92.4 |
| Redundancy | 2.9 | 1.8 |
| CC(1/2) | 0.990 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 40 % MPD, 5 % PEG 8000 and 0.1 M Sodium cacodylate pH 6.5 |
