6SAO
Structural and functional characterisation of three novel fungal amylases with enhanced stability and pH tolerance
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.015, 56.633, 166.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.760 - 1.200 |
| R-factor | 0.1109 |
| Rwork | 0.110 |
| R-free | 0.13410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.937 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rpim | 0.021 | 0.446 |
| Number of reflections | 150753 | 7123 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.8 | 96.7 |
| Redundancy | 7.6 | 4.6 |
| CC(1/2) | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | Natriumphosphate, Ammonium sulphate |
