6SA9
Endogenous Retrovirus HML2 Capsid NTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 42.387, 98.920, 76.508 |
| Unit cell angles | 90.00, 94.37, 90.00 |
Refinement procedure
| Resolution | 24.730 - 1.800 |
| R-factor | 0.1589 |
| Rwork | 0.157 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.977 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.050 | 0.034 | 0.245 |
| Total number of observations | 191173 | ||
| Number of reflections | 26836 | 2904 | 2066 |
| <I/σ(I)> | 27.1 | ||
| Completeness [%] | 92.2 | 98.2 | 71.4 |
| Redundancy | 7.1 | 7.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.2 M Sodium Acetate Trihydrate, 0.1 M Tris Hydrochloride and 15% (w/v) PEG 4000 |
