6SA8

ring-like DARPin-Armadillo fusion H83_D01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-16
Detector	PSI PILATUS 6M
Wavelength(s)	1.00000050167
Spacegroup name	C 2 2 21
Unit cell lengths	92.970, 145.460, 115.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.500 - 2.400
R-factor	0.2394
Rwork	0.237
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.500	46.515	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.369	0.068	4.495
Rmeas	0.377	0.069	4.579
Number of reflections	30897	396	2257
<I/σ(I)>	12.34	59.44	1.12
Completeness [%]	99.7	98.8	100
Redundancy	25.203	24.795	27.601
CC(1/2)	0.998	0.999	0.541

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	25 % PEG 2000 MME 0.2 M MgCl2 0.1M Tris HCl pH 8.5