6SA7

DARPin-Armadillo fusion C8long83

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99999815963
Spacegroup name	P 1 21 1
Unit cell lengths	54.470, 167.540, 95.030
Unit cell angles	90.00, 97.93, 90.00

Refinement procedure

Resolution	48.030 - 3.300
R-factor	0.2348
Rwork	0.233
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.030	48.030	3.390
High resolution limit [Å]	3.300	14.760	3.300
Rmerge	0.216	0.075	1.986
Rmeas	0.233	0.081	2.128
Number of reflections	24183	280	1864
<I/σ(I)>	6.75	20.53	1.03
Completeness [%]	95.2	94.3	99.1
Redundancy	7.44	6.95	7.723
CC(1/2)	0.995	0.991	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	4.3 M NaCl 0.1M HEPES NaOH pH 7.5