6SA6

DARPin-Armadillo fusion A5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-10
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	57.080, 51.560, 67.720
Unit cell angles	90.00, 95.24, 90.00

Refinement procedure

Resolution	45.560 - 1.600
R-factor	0.1832
Rwork	0.182
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.560	45.560	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.049	0.029	1.053
Rmeas	0.059	0.034	1.277
Total number of observations	159188
Number of reflections	49693	573	3432
<I/σ(I)>	13.01	40.42	1.17
Completeness [%]	95.7	92.4	90.3
Redundancy	3.203	3.133	2.995
CC(1/2)	0.998	0.996	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	18.6 % PEG 6,000 200 mM MgCl2 0.1M Tris HCl pH 8.1