6SA5
SALSA / DMBT1 / GP340 SRCR domain 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-01-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.816, 40.815, 62.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.300 - 1.290 |
| R-factor | 0.1563 |
| Rwork | 0.155 |
| R-free | 0.18840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a2e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.026 |
| Data reduction software | DIALS (xia2 Dials) |
| Data scaling software | DIALS (xia2 Dials) |
| Phasing software | MOLREP (CCP4Interface 7.0.056) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.820 | 1.310 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmeas | 0.122 | 1.193 |
| Number of reflections | 21958 | 2161 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 50 % dilution of 0.1 M LiSO4, 20 % (w/v) PEG6000, 0.01 M HEPES, pH 6.5 |
