6S9Y
Crystal structure of the N-terminal domain of the wild type parasitic PEX14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.925, 81.268, 39.287 |
| Unit cell angles | 90.00, 92.75, 90.00 |
Refinement procedure
| Resolution | 39.240 - 1.640 |
| R-factor | 0.1986 |
| Rwork | 0.197 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.630 | 1.740 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 28782 | 3967 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 96.2 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.998 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.10 M trisodium citrate pH 5.5, 40% PEG 600 |
