6S9F

Drosophila OTK, extracellular domains 3-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	C 2 2 21
Unit cell lengths	80.975, 189.909, 131.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.477 - 1.969
R-factor	0.2082
Rwork	0.207
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iep 2v9t 4of6
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3488)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.380	2.000
High resolution limit [Å]	1.969	1.970
Rmeas	0.083	0.835
Number of reflections	71214	6384
<I/σ(I)>	11	1.2
Completeness [%]	97.3	85.14
Redundancy	5.9	3.5
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.05 M HEPES (pH 7.0), 0.01 M magnesium chloride, 150 mM NDSB256 and 1.6 M ammonium sulfate