6S8U

Structure of the PfEMP1 IT4var13 DBLbeta domain bound to ICAM-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	142.410, 142.410, 224.225
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.205 - 3.670
R-factor	0.2425
Rwork	0.240
R-free	0.28650
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.205	3.730
High resolution limit [Å]	3.670	3.670
Number of reflections	15394	758
<I/σ(I)>	6.5
Completeness [%]	100.0
Redundancy	9.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Tris pH 8, 25% PEG 350 MME