6S8R
D. melanogaster RNA helicase Me31B in complex with GIGYF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9996 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.440, 41.960, 122.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.700 - 2.410 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5anr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.459 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 7712 | 522 |
| <I/σ(I)> | 8.91 | |
| Completeness [%] | 95.8 | 88.5 |
| Redundancy | 10.4 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium acetate (pH 5.0), 0.15 M ammonium chloride, 16% (w/v) PEG 6000 |
