6S7L
Arbitrium receptor from a Bacillus subtilis Katmira33 phage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.703, 209.765, 140.254 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.880 - 2.600 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6s7i |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.763 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.880 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 32018 | 4606 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 2-6 0.2 M Potassium acetate 0.1 M MES 6.0 15 % v/v Pentaerythritol ethoxylate (15/4 EO/OH) 3 % v/v Jeffamine T-403 |
