6S7G
Cfucosylated linker peptide SBL1 bound to Fucose binding Lectin LecB (PA-IIL) from Pseudomonas aeruginosa at 1.84 Angstrom resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-06-28 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.799998 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.230, 56.742, 70.622 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.428 - 1.841 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oxc |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.601 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.428 | 1.850 |
| High resolution limit [Å] | 1.841 | 1.841 |
| Rmeas | 0.286 | 1.580 |
| Number of reflections | 82590 | 13122 |
| <I/σ(I)> | 4.69 | 0.77 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 3.4 | 2.8 |
| CC(1/2) | 0.980 | 0.290 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.5 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol |
