6S68

Structure of the Fluorescent Protein AausFP2 from Aequorea cf. australis at pH 7.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	C 2 2 21
Unit cell lengths	54.410, 75.130, 100.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.110 - 2.060
R-factor	0.1683
Rwork	0.167
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	phiYFPv (Phialidium PDB:4HE4)
RMSD bond length	0.009
RMSD bond angle	1.690
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.110	2.110
High resolution limit [Å]	2.060	2.060
Rmeas	0.100
Number of reflections	13079	976
<I/σ(I)>	12.79	2.16
Completeness [%]	99.8	99.7
Redundancy	6	5.3
CC(1/2)	0.998	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	24% PEG 3350, 0.2 M sodium chloride, 0.1M HEPES pH 7.6