6S67
Structure of the Fluorescent Protein AausFP1 from Aequorea cf. australis at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.080, 101.410, 161.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.420 - 2.470 |
| R-factor | 0.1848 |
| Rwork | 0.182 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | phiYFPv (Phialidium PDB:4HE4) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.660 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.420 | 2.500 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmeas | 0.215 | |
| Number of reflections | 39086 | 2845 |
| <I/σ(I)> | 9.72 | 2.07 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.9 | 5.9 |
| CC(1/2) | 0.993 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.9 M trisodium citrate, 0.2 M sodium chloride, 0.1M Tris pH 7.0 |
