6S5X
Structure of RibR, the most N-terminal Rib domain from Group B Streptococcus species Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 48.880, 48.880, 144.573 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.305 - 1.700 |
| R-factor | 0.1613 |
| Rwork | 0.160 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.056 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.310 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.173 | |
| Number of reflections | 20225 | 1055 |
| <I/σ(I)> | 6.3 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.2 | 6.2 |
| CC(1/2) | 0.990 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 291 | 0.1 M citric acid pH 3.5, 2 M ammonium sulphate |
