6S5L
Anabaena Apo-C-Terminal Domain Homolog Of The Orange Carotenoid Protein In Native Conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.654, 112.276, 318.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.990 - 2.900 |
| R-factor | 0.2116 |
| Rwork | 0.209 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fej |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.712 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.990 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.060 | |
| Rmeas | 0.071 | |
| Rpim | 0.036 | |
| Number of reflections | 53339 | 7646 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.26M NaH2PO4, 0.14M K2HPO4 |
