6S5I

Structure of the Lausanne variant of myxoma virus M062 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 64
Unit cell lengths	94.827, 94.827, 44.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.410 - 2.450
R-factor	0.2169
Rwork	0.211
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cz3
RMSD bond length	0.014
RMSD bond angle	1.803
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.410	2.538
High resolution limit [Å]	2.450	2.450
Rmerge	0.054
Rmeas	0.057
Rpim	0.018
Number of reflections	8422	846
<I/σ(I)>	23.92
Completeness [%]	99.5	100
Redundancy	9.8
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris pH 7.5 50 mM CaCl2 6 - 8% PEG 8000