6S4S
CBDP35 Native structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 333.321, 95.118, 83.846 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 47.600 - 2.150 |
| R-factor | 0.18689 |
| Rwork | 0.186 |
| R-free | 0.21177 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6s3y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.611 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.560 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.115 | 0.980 |
| Number of reflections | 142267 | 14110 |
| <I/σ(I)> | 12.98 | 2.08 |
| Completeness [%] | 99.8 | 99.89 |
| Redundancy | 6.8 | 6.7 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 4M Sodium formate |
