6S4L
Structure of human KCTD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.77 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.454, 95.870, 104.343 |
| Unit cell angles | 90.00, 92.16, 90.00 |
Refinement procedure
| Resolution | 70.573 - 2.420 |
| R-factor | 0.2469 |
| Rwork | 0.245 |
| R-free | 0.28050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bxb |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.406 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.600 | 2.720 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Number of reflections | 30180 | 1509 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 59.7 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 12% PEG3350 -- 0.1M bis-tris-propane pH 7.8 -- 0.2M sodium iodide -- 10% ethylene glycol |
