6S46

Room temperature structure of the LOV2 domain of phototropin-2 from Arabidopsis thaliana 4158 ms after initiation of illumination, determined with a serial crystallography approach

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	293
Detector technology	PIXEL
Collection date	2017-11-10
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	P 43 21 2
Unit cell lengths	41.009, 41.009, 133.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.192 - 2.750
R-factor	0.2444
Rwork	0.242
R-free	0.29240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qqj
RMSD bond length	0.003
RMSD bond angle	0.651
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.010	2.900
High resolution limit [Å]	2.750	2.750
Number of reflections	3355	468
<I/σ(I)>	4.5	1.2
Completeness [%]	100.0	100
Redundancy	15	15.8
CC(1/2)	0.974	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12-17 % PEG8000 0.2 M Calcium acetate 0.1 M MES