6S3U
Adhesin P140 from Mycoplasma Genitalium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2012-05-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 459.190, 116.660, 285.640 |
| Unit cell angles | 90.00, 124.20, 90.00 |
Refinement procedure
| Resolution | 37.360 - 3.240 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rut |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 189.880 | 3.580 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Number of reflections | 123883 | 6159 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 63.5 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.986 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350 0.1M BisTris Propane pH7.5 0.2M Sodium Sulphate Hydrate |
