6S3T

P46, an immunodominant surface protein from Mycoplasma hyopneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	261.981, 185.010, 197.122
Unit cell angles	90.00, 134.43, 90.00

Refinement procedure

Resolution	29.000 - 3.500
R-factor	0.2424
Rwork	0.247
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rqg 6rnn
RMSD bond length	0.003
RMSD bond angle	0.631
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.300	3.600
High resolution limit [Å]	3.500	3.500
Number of reflections	83357	8278
<I/σ(I)>	5.51
Completeness [%]	98.8
Redundancy	1.9
CC(1/2)	0.981	0.292

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M MIB pH 4.5