6S3H
Crystal structure of helicase Pif1 from Thermus oshimai in complex with ADP-AlF4 and (dT)7ds11bp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.682, 200.130, 53.955 |
| Unit cell angles | 90.00, 93.44, 90.00 |
Refinement procedure
| Resolution | 29.510 - 2.060 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ftd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.860 | 2.131 |
| High resolution limit [Å] | 2.057 | 2.057 |
| Rmerge | 0.068 | 0.377 |
| Rmeas | 0.081 | 0.472 |
| Rpim | 0.034 | 0.279 |
| Number of reflections | 66828 | 5458 |
| <I/σ(I)> | 10.71 | 2.01 |
| Completeness [%] | 97.0 | 79.35 |
| Redundancy | 3.3 | 2.6 |
| CC(1/2) | 0.996 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Sodium Hepes-MOPS 0.1M CaCl2 0.03M MgCl2 0.03M PEG 8000 10% Ethylene glycol 20% |
