6S3F
Moringa seed protein Mo-CBP3-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 108.070, 108.070, 43.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.370 - 1.680 |
| R-factor | 0.18392 |
| Rwork | 0.181 |
| R-free | 0.23093 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.306 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.450 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.035 | 0.691 |
| Rpim | 0.020 | 0.411 |
| Number of reflections | 15156 | 741 |
| <I/σ(I)> | 24.7 | 2.8 |
| Completeness [%] | 99.6 | 95.5 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M citic acid 2.4M sodium formate |
