6S2Z

Water-soluble Chlorophyll Protein (WSCP) from Brassica oleracea var. Botrytis with Chlorophyll-b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-04-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	C 2 2 2
Unit cell lengths	86.360, 118.330, 38.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 2.500
R-factor	0.2207
Rwork	0.217
R-free	0.28360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hpz
RMSD bond length	0.009
RMSD bond angle	2.346
Data reduction software	XDS (VERSION Mar 15, 2019)
Data scaling software	XSCALE (VERSION Mar 15, 2019)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.720	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.180
Number of reflections	7149	526
<I/σ(I)>	15.82	3.78
Completeness [%]	99.5	99.6
Redundancy	7.51	7.51
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	10% PEG 3350, 0.1 M Ammonium dihydrogen phosphate, pH 4.5