6S2W

Structure of S. pombe Erh1, a protein important for meiotic mRNA decay in mitosis and meiosis progression.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2018-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	71.845, 123.847, 68.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.918 - 1.950
R-factor	0.202
Rwork	0.200
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w9g
RMSD bond length	0.010
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.918	45.918	2.070
High resolution limit [Å]	1.950	5.820	1.950
Rmerge	0.077	0.034	1.908
Rmeas	0.083	0.036	2.049
Total number of observations	176672
Number of reflections	22390	928	3501
<I/σ(I)>	14.26	47.84	1.02
Completeness [%]	99.6	98.8	98
Redundancy	7.891	7.099	7.549
CC(1/2)	0.999	0.999	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.3M Ammonium sulfate, 0.1M Sodium Acetate pH 3.8