6S2P

Structure of the NB-ARC domain from the Tomato immune receptor NRC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 64
Unit cell lengths	110.517, 110.517, 53.338
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.855 - 2.500
R-factor	0.3067
Rwork	0.305
R-free	0.33670
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.054	0.740
Number of reflections	13066	1463
<I/σ(I)>	24	3.5
Completeness [%]	100.0
Redundancy	10.3	10.3
CC(1/2)	1.000	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	8% PEG 1500, 0.1 M MMT (MES, malic acid and Tris,)