6S1L
Structure of fission yeast Mis16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.797, 123.604, 94.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.292 - 1.940 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cfs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15_3459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.920 | 2.009 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 33131 | 3286 |
| <I/σ(I)> | 8.38 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Na(OAc)2, 0.8 M NaH2PO4, 1.2 M KH2PO4, pH 4.5 |
