6S09

C-terminally extended and N-terminally truncated variant of FimA E. coli at 1.5 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	92.310, 105.230, 106.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.687 - 1.502
R-factor	0.1696
Rwork	0.169
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jty
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15-rc3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.687	47.687	1.540
High resolution limit [Å]	1.500	6.720	1.500
Rmerge	0.367	0.165	2.194
Rmeas	0.376	0.169	2.353
Total number of observations	2756146
Number of reflections	165865	2043	12125
<I/σ(I)>	5.87	19.36	0.87
Completeness [%]	100.0	99.7	99.9
Redundancy	16.617	24.023	7.571
CC(1/2)	0.992	0.986	0.276

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	28% PEG 8k, 0.1 M Di-Sodium Acetate pH 4.5 and 0.2 M Lithium Sulfate.