6RZQ
Plasmodium falciparum Hsp70-x chaperone nucleotide binding domain - ANP-PnP bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.290, 102.710, 103.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.470 - 1.810 |
| R-factor | 0.19381 |
| Rwork | 0.192 |
| R-free | 0.22403 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mkr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.491 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.260 | 1.840 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.057 | 1.523 |
| Rmeas | 0.062 | 1.650 |
| Rpim | 0.024 | 0.630 |
| Number of reflections | 78727 | 3870 |
| <I/σ(I)> | 12.65 | 1.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.5 | 6.7 |
| CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 24% w/v PEG 1500 20% v/v glycerol |
