6RZO

Crystal structure of the N-terminal carbohydrate binding module family 48 and ferulic acid esterase from the multi-enzyme CE1-GH62-GH10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 21
Unit cell lengths	66.595, 99.816, 114.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.638 - 1.632
R-factor	0.1713
Rwork	0.170
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jt2
RMSD bond length	0.009
RMSD bond angle	0.864
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.638	1.690
High resolution limit [Å]	1.632	1.632
Number of reflections	90065
<I/σ(I)>	7.09
Completeness [%]	94.4
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0 w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS propane pH 6.5, 200 mM sodium fluoride