6RZN
Crystal structure of the N-terminal carbohydrate binding module family 48 and ferulic acid esterase from the multi-enzyme CE1-GH62-GH10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-22 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.650, 99.900, 133.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.860 - 1.907 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jt2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.623 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.860 | 1.975 |
| High resolution limit [Å] | 1.907 | 1.907 |
| Number of reflections | 68057 | 6769 |
| <I/σ(I)> | 6.73 | |
| Completeness [%] | 98.5 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 50 mM ammonium sulfate, 50 mM Bis-Tris/HCl 6.5,30 % (v/v) pentaerythritol ethoxylate (15/4_EO/OH |
