6RYZ
SalL with S-adenosyl methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2018-04-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.928, 110.996, 163.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.000 - 1.500 |
| R-factor | 0.17989 |
| Rwork | 0.179 |
| R-free | 0.20324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q6i |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.717 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.120 | 0.810 |
| Rpim | 0.070 | 0.470 |
| Number of reflections | 149433 | 10925 |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 7.6 |
| CC(1/2) | 1.000 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1 M HEPES pH 7; 20% PEG 6000; 0.2 M CaCl2 |
