6RYW
Copper oxidase from Colletotrichum graminicola
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 171.407, 171.407, 171.407 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 121.200 - 2.600 |
| R-factor | 0.2237 |
| Rwork | 0.221 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c86 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.690 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.200 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.039 | 0.750 |
| Number of reflections | 25902 | 3166 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.4 | 21.7 |
| CC(1/2) | 0.998 | 0.587 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 291 | PEG 4000, sodium acetate, ammonium acetate |
