6RYV
Copper oxidase from Colletotrichum graminicola
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 172.111, 172.111, 172.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.920 - 2.300 |
| R-factor | 0.2408 |
| Rwork | 0.238 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c86 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.769 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.260 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.023 | 0.216 |
| Number of reflections | 37678 | 3667 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.4 | 24.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | PEG 2K MME, ammonium sulphate, Na acetate |
