6RYG
native structure of conglutinin carbohydrate recognition domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 43 |
| Unit cell lengths | 50.170, 50.170, 52.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 0.974 |
| R-factor | 0.1349 |
| Rwork | 0.135 |
| R-free | 0.13880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rym |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.654 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.170 | 52.247 | 1.020 |
| High resolution limit [Å] | 0.974 | 3.050 | 0.970 |
| Rmerge | 0.050 | 0.045 | 0.216 |
| Rmeas | 0.059 | 0.059 | 0.315 |
| Total number of observations | 471902 | 18257 | 4289 |
| Number of reflections | 61295 | 2374 | 1888 |
| <I/σ(I)> | 22.9 | 33 | 2.3 |
| Completeness [%] | 81.2 | 93.9 | 20.5 |
| Redundancy | 7.7 | 7.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 2.5 M ammonium sulfate, 0.1 M tris |
