6RYD

WUS-HD bound to TGAA DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97242
Spacegroup name	P 1 21 1
Unit cell lengths	55.740, 54.340, 75.560
Unit cell angles	90.00, 107.54, 90.00

Refinement procedure

Resolution	34.380 - 1.575
R-factor	0.189
Rwork	0.187
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ry3
RMSD bond length	0.006
RMSD bond angle	0.837
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.620	1.631
High resolution limit [Å]	1.575	1.575
Rpim	0.026
Number of reflections	59197	5914
<I/σ(I)>	12.14
Completeness [%]	98.8
Redundancy	3.4
CC(1/2)		0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium acetate (pH 4.6), 8% PEG 4000