6RYA

Structure of Dup1 mutant H67A:Ubiquitin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	116.328, 67.136, 182.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.070 - 2.210
R-factor	0.21265
Rwork	0.209
R-free	0.28877
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b7p
RMSD bond length	0.009
RMSD bond angle	1.682
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.070	2.289
High resolution limit [Å]	2.210	2.210
Rmerge	0.081	0.452
Rmeas	0.101	0.573
Rpim	0.060	0.347
Number of reflections	68825	7031
<I/σ(I)>	7.28
Completeness [%]	99.8
Redundancy	2.6
CC(1/2)	0.995	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	20 - 22.5 % PEG 3350/ PEG4000, 100 mM Tris-HCl pH 8.0, 100 mM Magnesium chloride