6RY0

Crystal structure of Dfg5 from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.972
Spacegroup name	C 1 2 1
Unit cell lengths	83.319, 54.989, 80.416
Unit cell angles	90.00, 90.27, 90.00

Refinement procedure

Resolution	30.280 - 1.050
R-factor	0.1069
Rwork	0.106
R-free	0.12280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.624
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.280	1.088
High resolution limit [Å]	1.050	1.050
Rmerge	0.028	0.316
Rmeas	0.035
Number of reflections	164339	16242
<I/σ(I)>	17.73	3.08
Completeness [%]	97.2	97.36
Redundancy	2.8	2.8
CC(1/2)	0.999	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291.15	Ammoniumiodide, PEG3350, Calcium acetate, MES, PEG 8000