6RY0
Crystal structure of Dfg5 from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.319, 54.989, 80.416 |
| Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
| Resolution | 30.280 - 1.050 |
| R-factor | 0.1069 |
| Rwork | 0.106 |
| R-free | 0.12280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.624 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.280 | 1.088 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.028 | 0.316 |
| Rmeas | 0.035 | |
| Number of reflections | 164339 | 16242 |
| <I/σ(I)> | 17.73 | 3.08 |
| Completeness [%] | 97.2 | 97.36 |
| Redundancy | 2.8 | 2.8 |
| CC(1/2) | 0.999 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | Ammoniumiodide, PEG3350, Calcium acetate, MES, PEG 8000 |
