6RXJ
Crystal structure of CobB wt in complex with H4K16-Acetyl peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 131.100, 131.260, 58.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.379 - 1.600 |
R-factor | 0.1736 |
Rwork | 0.173 |
R-free | 0.18760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s5p |
RMSD bond length | 0.008 |
RMSD bond angle | 1.111 |
Data reduction software | XDS (VERSION JAN 31, 2018) |
Data scaling software | XSCALE (VERSION JAN 31, 2018) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.379 | 46.379 | 1.640 |
High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
Rmerge | 0.068 | 0.033 | 1.618 |
Rmeas | 0.070 | 0.035 | 1.682 |
Total number of observations | 868617 | ||
Number of reflections | 66812 | 839 | 4841 |
<I/σ(I)> | 18.83 | 57.82 | 1.6 |
Completeness [%] | 99.9 | 99.5 | 99.5 |
Redundancy | 13.001 | 12.035 | 13.17 |
CC(1/2) | 0.999 | 0.999 | 0.644 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1M Bis-Tris, 0.03 M HCl, 22% PEG3350 |