6RXG
Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Complex with Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.600, 72.760, 87.330 |
| Unit cell angles | 71.43, 72.21, 76.75 |
Refinement procedure
| Resolution | 68.231 - 1.710 |
| R-factor | 0.1489 |
| Rwork | 0.147 |
| R-free | 0.19360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rxd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.927 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.231 | 1.740 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.063 | 0.525 |
| Number of reflections | 123847 | 6108 |
| <I/σ(I)> | 7 | 1.4 |
| Completeness [%] | 95.5 | 95.3 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M MES, 0.01 M zinc chloride, 18 % PEG 6000 |
