6RXD
Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Apo Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.660, 72.940, 88.160 |
| Unit cell angles | 71.61, 72.10, 77.17 |
Refinement procedure
| Resolution | 46.528 - 1.650 |
| R-factor | 0.1544 |
| Rwork | 0.153 |
| R-free | 0.17710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fdt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.008 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.059 | 0.450 |
| Number of reflections | 141139 | 6851 |
| <I/σ(I)> | 19.7 | 1.8 |
| Completeness [%] | 95.5 | 94.8 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M MES, 0.01 M zinc chloride, 18 % PEG 6000 |
