6RWV
Structure of apo-LmCpfC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.303, 76.723, 52.258 |
| Unit cell angles | 90.00, 106.56, 90.00 |
Refinement procedure
| Resolution | 50.091 - 1.639 |
| R-factor | 0.177332910511 |
| Rwork | 0.176 |
| R-free | 0.20130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hk6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.934 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2719) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.091 | 1.698 |
| High resolution limit [Å] | 1.639 | 1.639 |
| Rmerge | 0.064 | 0.682 |
| Rmeas | 0.091 | 0.965 |
| Rpim | 0.064 | 0.682 |
| Number of reflections | 44834 | 4416 |
| <I/σ(I)> | 6.5 | 1.09 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.997 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 16% w/v PEG 8000, 20% w/v Glycerol, 0.04 M KH2PO4 |
